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KMID : 0380519970010020119
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Korean Journal of Veterinary Pathology
1997 Volume.1 No. 2 p.119 ~ p.123
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Digoxigenin-labeled in situ hybridization, Aujeszky’s disease
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Kim Soon-Bok
Moon Oun-Kyong
Song Keun-Seog
Yeo Sang-Geon
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Abstract
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The objective of this study was to develop digoxigenin (DIG)-labeled in situ hybridization (ISH) test for diagnosis of Aujeszky’s Disease(AD) in infected organs. Specific DNA with well conserved gene sequences encoding gp50 antigen in AD virus (ADV) was obtained by Polymerase Chain Reaction (PCR) method. A pair of oligonucleotide primers used in PCR allowed amplification of a 217 bp sequence from the gp50 ADV gene. The DNA was then labeled with DIG by primer labeling method for use as probe in ISH test to detect ADV nucleic acids in various tissue. Positive hybridization was demonstrated by dark pigmentation in nuclei and cytoplasm of ADV infected cells particularly in brain tonsillar crypt epithelium and pulmonary alveolar cells. This result suggests that ISH is a valuable sensitive and rapid diagnostic test for AD.
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KEYWORD
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Digoxigenin-labeled in situ hybridization, Aujeszky’s disease
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